Study of the fibrinolytic activity of serrapeptase and its in vitro thrombolytic effects

Serrapeptase, a proteolytic enzyme, has been used for the adjuvant treatment of many diseases. However, its fibrinolytic activity is still uncertain. Herein, serrapeptase and in vitro thrombolytic effects were investigated. The results showed that was 1295 U/mg, specific 3867 U/mg protein when toward casein 2800 U/mg. optimum temperature pH 37-40°C 9.0, respectively. At 1 mmol/L, Zn2+, Mn2+ Fe2+ could activate serrapeptase, while K+, Cu2+, sodium dodecyl sulfate (SDS) ethylene diamine tetraacetic acid (EDTA) inhibited it. In tests completely prevent blood coagulation at 150 U/mL, percentage clot lysis reached 96.6% 37°C after 4 h 300 U/mL. These indicate excellent activity, can be as health food or candidate drug prevention thrombotic